In vivo processing and antibiotic activity of microcin B17 analogs with varying ring content and altered bisheterocyclic sites
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چکیده
منابع مشابه
In vivo processing and antibiotic activity of microcin B17 analogs with varying ring content and altered bisheterocyclic sites.
BACKGROUND The Escherichia coli peptide antibiotic microcin B17 (MccB17) contains four oxazole and four thiazole rings, and inhibits DNA gyrase. The role of individual and tandem pairs of heterocycles in bioactivity has not been determined previously. RESULTS The two tandem 4,2-bisheterocycles in MccB17 were varied by expression of MccB17 or mutants containing altered sequences at Gly39-Ser40...
متن کاملIn vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites.
Microcin B17 (MccB17) is a 3.1-kDa Escherichia coli antibiotic that contains thiazole and oxazole heterocycles in a peptide backbone. MccB17 inhibits its cellular target, DNA gyrase, by trapping the enzyme in a complex that is covalently bound to double-strand cleaved DNA, in a manner similar to the well-known quinolone drugs. The identification of gyrase as the target of MccB17 provides an opp...
متن کاملPosttranslational heterocyclization of cysteine and serine residues in the antibiotic microcin B17: distributivity and directionality.
To produce the antibiotic Microcin B17, four Cys and four Ser residues are converted into four thiazoles and four oxazoles by the three subunit Microcin B17 synthetase. High-resolution mass spectrometry (MS) was used to monitor the kinetics of posttranslational heterocyclic ring formation (-20 Da per ring) and demonstrated the accumulation of all intermediates, from one to seven rings, indicati...
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15 صفحه اولExpressed protein ligation to probe regiospecificity of heterocyclization in the peptide antibiotic microcin B17.
BACKGROUND The Escherichia coli peptide antibiotic microcin B17 (MccB17) contains thiazole and oxazole heterocycles derived from a distributive yet directional cyclization of cysteines and serines in the McbA precursor catalyzed by MccB17 synthetase. Whether the formation of upstream rings potentiates downstream heterocyclization has not been previously determined. RESULTS McbA fragments (46-...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 1999
ISSN: 1074-5521
DOI: 10.1016/s1074-5521(99)80076-3